Relationship between Sperm Vacuoles, Morphology, DNA Damage, Chromatin State, and Oxidative Stress
ACOG ePoster. Tran M.
Apr 27, 2018; 212146
Mai Tran
Mai Tran
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Abstract
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Introduction: Some studies found that the presence of sperm nuclear vacuoles is positively correlated with DNA fragmentation. Our objectives are: 1) Evaluate the quantity and size of vacuoles and their association with morphology, DNA damage, and chromatin compaction in semen from men who underwent assisted reproductive treatments; and 2) Investigate the effect of oxidative stress on DNA fragmentation.

Methods: Semen samples from 75 men were analyzed and the number and size of vacuoles, DNA fragmentation, and chromatin compaction state were determined via MSOME, TUNEL, and acridine orange (AO) respectively. Furthermore, the level of vitality, DNA fragmentation, and chromatin compaction were evaluated prior to and after 4 hours of oxidative stress (H202).

Results: In the Kruger≥14% population, spermatozoa with one or more vacuoles that covered 20% of the nuclear surface had more DNA fragmentation compared to those that covered less. Also, spermatozoa with 1 large or several vacuoles had a chromatin compaction state that was more susceptible to acid-induced DNA denaturation compared to those with other vacuoles pattern. In the Kruger
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