Is There a Synthetic Lethality Between FANCM-U and SMARCAL1 ?
ACOG ePoster. Sabet L. Apr 27, 2018; 211769; 27A
Liliya Sabet
Liliya Sabet
Login now to access Regular content available to all registered users.

You may also access this content "anytime, anywhere" with the Free MULTILEARNING App for iOS and Android
Rate & Comment (0)
Introduction: Our oncology lab recent results showed that there is a strong synthetic lethal interaction between BRCA1 and FANCM. We observed a similar synthetic lethal interaction between BLM, a DNA helicase, and FANCM, albeit somewhat weaker than that of BRCA1 and FANCM. Furthermore we showed that BRCA1 recruits BLM to the replication stress sites induced by the depletion of FANCM. Our data suggest that a FANCM inhibitor may be used to potentially kill cancers with BRCA1 mutations. Most recently another DNA helicase, SMARCAL1, was shown to resolve replication stress.

Methods: We proposed to test whether there is a synthetic lethal interaction between SMARCAL1 and FANCM-U by performing a Crystal Violet Cell Viability Assay. We transfected U2-OS, a human osteosarcoma cell line, with siRNA targeting Luciferase (serves as the negative control siRNA), FANCM-U, SMARCAL1, and both FANCM-U and SMARCAL1. Then we seeded 4,000 cells per well on a 12-well plate. 7 to 10 days later we stained the cells with Crystal Violet, a blue dye, to visualize viable cells. Finally we quantitated the amount of viable cells with a spectrometer. We repeated the Crystal Violet Cell Viability Assay three times to obtain a reproducible result.

Results: Based on spectrometer results the amount of viable cells or intensity of light detected in the control and FANCM-U and SMARCAL1 wells were similar.

Conclusion/Implications: This study did not show synthetic lethal interaction between FANCM-U and SMARCAL1. Further research is ongoing to support or oppose our hypothesis.

    This eLearning portal is powered by:
    This eLearning portal is powered by MULTIEPORTAL
Anonymous User Privacy Preferences

Strictly Necessary Cookies (Always Active)

MULTILEARNING platforms and tools hereinafter referred as “MLG SOFTWARE” are provided to you as pure educational platforms/services requiring cookies to operate. In the case of the MLG SOFTWARE, cookies are essential for the Platform to function properly for the provision of education. If these cookies are disabled, a large subset of the functionality provided by the Platform will either be unavailable or cease to work as expected. The MLG SOFTWARE do not capture non-essential activities such as menu items and listings you click on or pages viewed.

Performance Cookies

Performance cookies are used to analyse how visitors use a website in order to provide a better user experience.

Save Settings